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ABSTRACT The present update on the global distribution of Mycobacterium tuberculosis complex spoligotypes provides both the octal and binary descriptions of the spoligotypes for M. Tuberculosis complex, including Mycobacterium bovis, from >90 countries (13,008 patterns grouped into 813 shared types containing 11,708 isolates and 1,300 orphan patterns). A number of potential indices were developed to summarize the information on the biogeographical specificity of a given shared type, as well as its geographical spreading (matching code and spreading index, respectively). To facilitate the analysis of hundreds of spoligotypes each made up of a binary succession of 43 bits of information, a number of major and minor visual rules were also defined. A total of six major rules (A to F) with the precise description of the extra missing spacers (minor rules) were used to define 36 major clades (or families) of M. Some major clades identified were the East African-Indian (EAI) clade, the Beijing clade, the Haarlem clade, the Latin American and Mediterranean (LAM) clade, the Central Asian (CAS) clade, a European clade of IS 6110 low banders (X; highly prevalent in the United States and United Kingdom), and a widespread yet poorly defined clade (T).

When the visual rules defined above were used for an automated labeling of the 813 shared types to define nine superfamilies of strains ( Mycobacterium africanum, Beijing, M. Bovis, EAI, CAS, T, Haarlem, X, and LAM), 96.9% of the shared types received a label, showing the potential for automated labeling of M. Tuberculosis families in well-defined phylogeographical families. Intercontinental matches of shared types among eight continents and subcontinents (Africa, North America, Central America, South America, Europe, the Middle East and Central Asia, and the Far East) are analyzed and discussed. Tuberculosis (TB) remains a major killer, with >8 million new cases and >2 million deaths each year.

TB control essentially relies on improvement of expanded and reliable local microbiological diagnostic capacities, the availability of drugs on a worldwide basis, and compliance through adequate treatment strategy. Other related problems include the AIDS epidemic, less access to health services in countries needing it badly, and increasing multidrug resistance. Due to increased human migration from high-prevalence areas, there may also be a danger of the spread of multidrug-resistant TB and consequently a need for earlier detection of new outbreaks (). A better knowledge of moving and expanding clones, such as Beijing (, ), that may harbor various degrees of virulence () is also urgently needed. Warcraft 3 Reign Of Chaos Torrent Iso Ps2.

In this context, the genotyping of tubercle bacilli and the study of the virulences of various clones from different settings may help to better define TB control measures. Complementary to traditional epidemiology, molecular epidemiology based on PCR fingerprinting methods, such as spacer oligonucleotide typing (spoligotyping) (), has emerged as a fast, reliable, and cost-effective alternative to traditional IS 6110 restriction fragment length polymorphism (RFLP) fingerprinting. Based on the variability of the direct-repeat (DR) locus (,,,,, ), spoligotyping is useful both for tracking epidemics (,,,,, ) and to detect new outbreaks and better define high-risk populations in order to focus prevention strategies on the subpopulations that need them most (). In this context, the construction of polymorphism databases constitutes a powerful tool for studying the epidemiology and evolutionary genetics of tubercle bacilli () and, ultimately, the mechanisms of genetic variability by using data-mining methods (). However, previous databases were only poorly representative of the worldwide diversity of Mycobacterium tuberculosis genomes (,, ), e.g., the previously published 259 shared types (identical spoligotypes shared by two or more patient isolates; available online at ) contained more than two-thirds of the isolates from Europe and the United States.

Nonetheless, these initial studies supported the fact that a significant number of M. Tuberculosis isolates are confined to specific geographic locations (,, ). In this article, we describe an update on the global distribution of M. Tuberculosis complex spoligotypes (SpolDB3.0). With a total of 13,008 patterns from >90 countries, the SpolDB3.0 database contains both the octal () and binary () descriptions of the spoligotypes for all the current M. Tuberculosis complex members ( M.

Tuberculosis, Mycobacterium africanum, Mycobacterium bovis, Mycobacterium microti, Mycobacterium canetti, and Mycobacterium caprae). It also better describes the diversity of TB genotypes, since the 24 most prevalent alleles now represent only 53% of the total instead of 65%, as in a previous study (). Similarly, the computation of the genetic diversity index ( H []) gives an H of 97.4% with the current version of the database compared to 93% with the previous version of the database. Visual rules for defining major spoligotyping families.Simultaneous analysis of a visual pattern made up of a binary succession of information of 43 bits is not an easy task for the human brain. Octal numbering () is an improvement for database storage but has not yet proven useful for taxonomic and phylogenetic analysis.

Moreover, previous work using mathematical modeling has shown that not all spacer positions in a spoligotype carry the same amount of information (). Consequently, in order to better recognize patterns visually, we found it convenient to define six major visual rules as follows: rule A, absence of sp29 to -32, presence of sp33, and absence of sp34; rule B, absence of sp21 to -24 and sp33 to -36; rule C, absence of sp18 and sp33 to -36; rule D, absence of sp39 to -43; rule E, absence of sp31 and sp33 to -36; rule F, absence of sp33 to -36. These six major rules were used together with the precise description of the extra missing spacers (minor rules) to define a total of 36 major clades of circulating M. Tuberculosis isolates (; ). Some major clades identified are the Beijing clade; the East African-Indian (EAI) clade; the Haarlem clade; the Latin American and Mediterranean (LAM) clade; the Central Asian (CAS) clade; a European clade of IS 6110 low banders, i.e., isolates containing ≤4 copies of the IS 6110 element (X; highly prevalent in the United States and United Kingdom); and a widespread yet poorly defined clade (T) characterized by the absence of sp33 to -36. Global distribution of the shared types.The 13,008 spoligotype patterns were grouped into 813 shared types containing 11,708 (90%) of the isolates and 1,300 (10%) orphan patterns (clinical isolates showing unique spoligotypes). Since the publication of the previous database (), the number of clustered isolates (shared types) has increased from 84 (2,779 of 3,319) to 90% (11,708 of 13,008).

An identical clustering rate was found in the largest spoligotyping study published so far, which was performed in Texas and totaled 1,283 patients (). The distribution of the shared types, their respective sizes, and their relative distributions in different locations are summarized in Fig.. The 20 most frequent types among the 813 shared types totaled 5,865 clinical isolates, i.e., 50% of all the clustered isolates. Three of these profiles correspond to M. Bovis: types 683 and 481 for M. Bovis and type 482 for M.

The addition of the next 30 most frequent spoligotypes slightly increased the total number of shared types assessed (65% instead of the initial 50%). SpolDB3.0 better describes the diversity of TB genotypes, since the 24 most prevalent alleles now represent only 53% of the total instead of 65% as in the previous study (). The computation of the genetic diversity index () is done using the formula H = 2 n(1 − Σ x i 2)/2 n − 1, where H is the genetic diversity index, x is the frequency of the allele i, and n is the population size, giving an H value of 97.4% with the current version of the database.

An identical calculation with the previous version gave an H value of 93% (). In Fig., which depicts the 20 most frequent spoligotypes, the Beijing type (ST 1) is the most frequent (1,282 isolates, or 11% of all clustered isolates), followed by the Haarlem type (ST 47 and ST 50, representing ∼6% of all clustered isolates).

The newly designated X1 and X2 spoligotypes (ST 119 and ST 137), which tend to be highly prevalent in the United Kingdom and the United States, represent 6.4% of the clustered isolates. Figure shows that one-third of all the shared types consist of two isolates only. This result suggests an important local diversity of spoligotyping. Nonetheless, a match of two identical but rare spoligotypes found in a single setting is different from a match found by database comparison of two widely separated isolates.

The first case may be an early indicator of clonal expansion and ongoing transmission, whereas the second case, depending on the spoligotype, is likely to be due to homoplasy (independent acquisition of two similar structures without common ancestors). Alternatively, such matches, whether near extinction or not, may also reflect past epidemiological events. Figure shows that one-third of the shared types are repeatedly found within a single geographic area. This result corroborates the observations from Fig. And suggests that spoligotyping performed as a single genotyping method in a new setting may be a good indicator of strain identity and helps to produce a precise picture of epidemiologically important clones. Matching analysis of shared types found in one or two geographic locations.Table shows the results of matching analysis of shared types which have been reported in one or two continental regions as defined in Materials and Methods ( n = 625).

This analysis demonstrates that the diversity of clustered spoligotypes is high within Europe ( n = 163), the United States ( n = 119), and Africa ( n = 53). When the sample size is normalized, the diversity, limited to a specific continent (the number of shared types limited to a given continent divided by the total number of isolates within the continent), appears to be highest within Europe ( n = 163 of 3,927, or 0.041), followed by Africa ( n = 53 of 1,303, or 0.039) and the United States ( n = 119 of 5,157, or 0.023). On the other hand, the lowest diversity is found in the Far East ( n = 9 of 757, or 0.011).

The greatest number of intercontinental matches is found between North America and Europe ( n = 88). This class is made up of clones that are likely to represent, at least partly, historical TB transmission events between Europe and the United States, a phenomenon that may be explained either by the relatively old demographic links between these two continents or by recent transmission from identical high-prevalence countries. A significant number of these intercontinental matches are also found between Africa and Europe ( n = 22), South America and Europe ( n = 17), Central America and Europe ( n = 13), the Middle East and Europe ( n = 13), and, to a lesser extent, between Far East Asia and North America ( n = 12). These data should be interpreted in the light of old, as well as recent, migratory flux and deserve further study. Among the matches between Europe and the Middle East and Central Asia, a majority concerned IS 6110 low banders from the United Kingdom that are known to be linked to the EAI clade of M. Tuberculosis (, ). However, the recent finding of a spoligotype harboring a typical EAI signature (sp29 to -32 with sp34 missing) in a sample from 15th-century M.

Tuberculosis DNA found in the Wharram Percy medieval village in the United Kingdom is controversial as far as the precise origin of the EAI clade (). Snapshot of moving and expanding clones of M. Tuberculosis and their global distribution.In a previous report on 259 shared types observed for 3,319 isolates from 47 countries, at least six major clades of tubercle bacilli were described (). The present study permitted us to define a total of 36 potential superfamilies of spoligotypes using visual major rules A to F, defined in Materials and Methods, and a number of minor rules available online. Automated Excel labeling of the whole database ( n = 813 shared types) following the visual rules defined above, and on a total of nine superfamilies of strains ( M.

Africanum, Beijing, M. Bovis, EAI, CAS, T group of families, Haarlem, X family, and LAM family), resulted in the labeling of 788 of 813 (96.9%) shared types. These results should be further assessed and generalized using data-mining methods (). The distribution of the most frequently observed spoligotypes, schematized in Fig., underlined some major differences among the continental regions studied, e.g., the number of orphan types (or singletons) ranged from a low of 8% (North America) to a high of 21% (Middle East and Central Asia). Similarly, minor shared types ranged from 12% in the Far East to >50% in Europe and the Middle East and Central Asia.

Among major clades, the heterogeneity of the distribution of the Beijing type (type 1 in the database) was noteworthy: it ranged from. DISCUSSION Some recent papers have dealt with the construction of spoligotyping databases (,, ).

() described a study of 1,429 M. Tuberculosis isolates from 1,283 patients as part of an ongoing population-based TB epidemiology study in Houston, Tex. This paper was soon followed by a report of the biogeographical distribution of 3,319 spoligotype patterns and 259 shared types from 47 countries worldwide (). The first study essentially focused on isolates from patients residing in a single state in the United States, whereas in the second study, >73% of the isolates described were from Europe and the United States.

Despite these limitations, the studies underlined the fact that a significant number of M. Tuberculosis isolates in circulation were essentially confined to specific geographic locations (, ). By including new spoligotyping data from all over the world, SpolDB3.0 has increased the overall representation; nonetheless, a more representative description of the worldwide diversity of tubercle bacilli should be possible through the acquisition of information from Asian and African countries. The construction of global polymorphism databases constitutes a powerful tool, as it permits a quantitative estimation of the measure of DNA variations at the chromosomal level by the number of genetic structures observed so far. Similarly to what is done in Drosophila melanogaster population genetics, where inversions have been classified as “common ubiquitous, rare endemic, recurrent endemic and unique endemic” (), we attempted to categorize most of the geographic variations in the DR loci observed so far by spoligotyping, so as to have a better knowledge of moving and expanding clones of M. For this purpose, we introduced new indices (MC and SI) and qualifiers (C1 and C2) in order to better describe the spatiotemporal status of natural populations of the M.

Tuberculosis complex. For the spatial distribution, the populations studied were defined as endemic, localized, or ubiquitous. For the quantitative distribution, the populations were defined as epidemic, common, recurrent, or rare. These definitions synthetically define a spatiotemporal status for each shared type and, together with its genetic structure, may provide a global idea of its evolutionary history.

The results obtained also underline the well-known fact that casual contacts and sporadic cases, although difficult to detect, are responsible for most of the microepidemics and constitute an important means of TB transmission (). Our next objective is to better describe the genetic diversity of the M. Tuberculosis complex worldwide, which may be achieved by recruitment of adequate clinical isolates or DNA samples or inclusion of representative spoligotyping data in the database. Construction of new mathematical models that permit an interpretation based on the combination of DNA fingerprinting, epidemiological, and demographical data should further improve our knowledge of evolutionary processes that intervene in the development and spread of infectious diseases. Regarding the genetic variability of the DR locus, it was recently shown to be a part of a larger family of sequence repeats among prokaryotes ().

Much remains to be done to precisely define the potential phylogenetic links within various alleles of this locus, as well as to investigate potential links that are found across individual studies targeting local epidemiological issues, particularly since TB does not respect man-made frontiers. Little is also known about the microevolutionary events associated with the DR locus and how they may influence the interpretation of both spoligotyping and IS 6110 RFLP data (). Indeed, different isolates from the same strain family and isolates from different strain families may rarely converge to give the same spoligotype pattern (). Though of limited importance, this bias may be investigated in detail in future by using second-generation spoligotyping based on a set of new spacer oligonucleotides () or by assessment of other genetic markers () in selected strains.

The management of such projects will be facilitated by automation of data entry and data mining to further update SpolDB3.0 (, ). The data acquisition, similarity search, and matching process; labeling; and translation from binary to octal format and vice versa are already automated, and future data exchange and internet working of SpolDB3.0 with other databases (such as IS 6110 RFLP or mycobacterial interspersed repetitive units) should soon allow new queries to be screened against an updated version. The facility by which detection of matches between potentially linked strains can be achieved may make SpolDB3.0 a new tool for international studies of TB transmission.

Indeed, the detection of a match between two rare profiles in SpolDB3.0 may be a start to gathering complementary genotyping information, such as IS 6110 RFLP or polymorphic GC-rich-sequence RFLP in other international databases, to demonstrate clonality of the studied isolates () and to detect unsuspected epidemiological links. In conclusion, SpolDB3.0 constitutes a potential tool for global TB epidemiology and population genetics and M. Tuberculosis complex taxonomy and phylogeny. It underlines major differences in the population structures of tubercle bacilli within the eight subcontinents studied, and by using new indices and qualifiers, it has led to better interpretation methods and the possibility of future comparison with other methods, such as mycobacterial interspersed repetitive units (). Nevertheless, further work is still needed to get a more exhaustive global picture of worldwide tubercle bacillus genetic variability. Another major issue will be the ability to link this genetic diversity to virulence and/or fitness factors and ultimately to the genetic predisposition factors of the human or animal hosts. Driver For Flash Drive Staples 256mb Ati.